Volume 8 ; Issue 2 ; in Month : July-Dec (2025) Article No : 172
Khan S, Pathan IK, Jamal R, et al.

Abstract

Aim: This study evaluates the therapeutic potential of Clitoria ternatea (White flower plant) ethanolic leaves extract using an in-silico approach, focusing on its antioxidant and Anti-Inflammatory Properties. Materials and Methods: Ethanolic extraction was performed using the Soxhlet method, followed by qualitative analysis, including phytochemical, FT-IR, and GC-MS analyses, as well as total flavonoid, total phenolic, and terpenoid content analyses. Predict the therapeutic potentials of the extract with PDB ID: 3VLN (Antioxidant), PDB ID: 2AZ5 (Anti-inflammatory), and validate the in-silico study by performing the respective in vitro study. Results: Flavonoids, anthocyanins, saponins, phenolic compounds, and terpenoids are bioactive compounds in the extract, and their functional groups are reported by phytochemical tests and FTIR, respectively. GC-MS identifies 43 different molecules. Total flavonoid content is 0.0153 mg of Quercetin QUE /gm extract, total phenolic 0.0182 of GAE/gm extract, and terpenoid content is 96.5%. Molecular docking study predicted 14 molecules for antioxidant activity, 14 molecules for anti-inflammatory activity, and 21 molecules for antimicrobial activity. Extract and ascorbic acid % Radical Scavenging Assay at 300 µg/mL are 57.16±0.48 and 39.15±0.62%, respectively. The IC₅₀ values for the extract and ascorbic acid are 171.35 µg/mL and 120.81 µg/mL, respectively. The HRBC membrane stabilization assay showed % Protection at concentrations of 1000 μg/ml of extract (53.12±0.82%) and Aspirin (85.13±0.87%). Conclusion: The present research focuses on the therapeutic properties of Clitoria ternatea (white flower) ethanolic leaf extract, which is abundant in flavonoids, phenolics, and terpenoids. GC-MS, molecular docking, ADMET, and in vitro studies indicate its antioxidant and anti-inflammatory properties, indicating potential use in phytopharmaceuticals and functional food development.

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