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<title>International-Journal-of-Biomedical-Investigation-ISSUE VOLUME Volume 3 ISSUE Issue 1</title>
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<description>
International-Journal-of-Biomedical-Investigation: VOLUME Volume 3 ISSUE Issue 1, Jan-June 2020
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<language>en-us</language>
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<title>International-Journal-of-Biomedical-Investigation-ISSUE VOLUME Volume 3 ISSUE Issue 1</title>
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		<title>Identification-of-Novel-Proteins-Interacting-with-Proprotein-Convertase-SubtilisinKexin-9</title>
		<pubDate>08-Mar-2020</pubDate>
<link>http://ijbi.edwiserinternational.com/admin/uploads/D5gJhC.pdf</link>
		<author>Melendez-QM-Wooten-CJ-Krishnaji-ST-et-al</author>
		<comments>{http://www.edwiserinternational.com/contact-us.php}</comments>
		<category>Pharmaceutical Science,Medical Science</category>
		<description>{<![CDATA[High levels of cholesterol, especially as low-density lipoprotein (LDL), are a well-known risk factor for atherosclerotic-related diseases. The key atherogenic property of LDL is its ability to form atherosclerotic plaque. Proprotein convertase subtilisin/kexin-9 (PCSK9) is an indirect regulator of plasma LDL levels by controlling the number of LDL receptor molecules expressed at the plasma membrane, especially in the liver. Herein, we performed a combination of affinity chromatography, mass spectrometry analysis and identification, and gene expression studies to identify proteins that interact with PCSK9. Through these studies, we identified three proteins, alpha-1-antitrypsin (A1AT), alpha-1-microglobulin/bikunin precursor (AMBP), and apolipoprotein H (APOH) expressed by C3A cells that interact with PCSK9. The expression levels of A1AT and APOH increased in cells treated with MITO+ medium, a condition previously shown to affect the function of PCSK9, as compared to treating with Regular (control) medium. However, AMBP expression did not change in response to the treatments. Additional studies are required to determine which of these proteins can modulate the expression/function of PCSK9. The identification of endogenous modulators of PCSK9s function could lead to the development of novel diagnostic tests or treatment options for patients suffering hypercholesterolemia in combination with other chronic metabolic diseases.]]>}</description>
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		<title>Antibacterial-Activity-of-Tamarindus-indica-Fruit-Extracts-against-Staphylococcus-aureus-and-Escherichia-coli</title>
		<pubDate>11-Apr-2020</pubDate>
<link>http://ijbi.edwiserinternational.com/admin/uploads/9Tk0Bb.pdf</link>
		<author>Phiri-M-Mufwambi-W-Mudenda-S-et-al</author>
		<comments>{http://www.edwiserinternational.com/contact-us.php}</comments>
		<category>Pharmaceutical Science,Medical Science</category>
		<description>{<![CDATA[Staphylococcus aureus and Escherichia coli cause a multitude of mild to serious infections in humans. Microorganisms such as Staphylococcus aureus and Escherichia coli have either developed resistance against the current treatments, or the current treatments cause unacceptable side effects. It is therefore imperative that research into new potential treatments is prioritized. Plants have shown to be a promising source of antibacterial agents. This study, therefore, aimed to determine the antibacterial activity of aqueous and ethanolic extracts of Tamarindus indica against Staphylococcus aureus and Escherichia coli. This was an in-vitro laboratory-based experimental study. Staphylococcus aureus and Escherichia coli were cultured in the laboratory. Different concentrations of aqueous and ethanolic extracts of Tamarindus indica were tested for antibacterial activity using the disc diffusion method. The sensitivity of the tested microorganisms to aqueous and ethanolic plant extracts was shown by zones of inhibition after incubation. The aqueous and ethanolic extracts of Tamarindus indica exhibited activity against S. aureus and E. coli in a dose-dependent manner. The minimum inhibitory concentration was found to be 0.5 mg/ml against both micro-organisms. E. coli was more susceptible to both T. indica extracts with the 100 mg/ml dose giving a zone of inhibition of 14.8 mm  0.3 with the aqueous extract, and 13.5 mm  0.5 with the ethanolic extract. Testing of 100 mg/ml aqueous and ethanolic T. indica extracts against S. aureus resulted in maximum zones of inhibition of 8.5 mm  0.5. Tamarindus indica possesses dose-dependent antibacterial activity against Escherichia coli and Staphylococcus aureus.]]>}</description>
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		<title>Berberine-verses-Sorafenib-for-the-treatment-of-Hepatocellular-carcinoma</title>
		<pubDate>26-Apr-2020</pubDate>
<link>http://ijbi.edwiserinternational.com/admin/uploads/nxwO57.pdf</link>
		<author>Phiri-M-Duo-L-Tengfei-L-et-al-</author>
		<comments>{http://www.edwiserinternational.com/contact-us.php}</comments>
		<category>Pharmaceutical Science,Medical Science</category>
		<description>{<![CDATA[Traditional Chinese medicine as Complimentary Alternative Medicine (CAM) is useful in the treatment of chronic diseases including Cancer. Studies have shown components in plants responsible for their synergic effect and, analysis has revealed multiple components of plants that work synergistically to produce useful toxic effect against hepatocellular carcinoma of which a single conventional drug cannot produce. Different types of medicines have been discovered for HCC, but, a compiled quantitative analysis of these compounds will influence clinicians and researchers decisions about their use, research methodology to yield new drugs and most importantly, it can reduce research cost and time. 80% of the general population in the world are using plants to treat several illnesses and about 25% of the drugs prescribed worldwide come from plants. Sorafenib has been the drug of choice for over 10 years for the treatment of Hepatocellular Carcinoma patients of which Berberine a Traditional Chinese Medicine has also shown to be effective. This review in campuses articles obtained from western and Chinese journal data base. In conclusion, TCM has a vast number of components that can be used as alternative novel compounds in cancer treatment. Alkaloid Berberine has components that can be utilized to circumvent Hepatocellular Carcinoma when used in combination.]]>}</description>
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